The COI DNA Barcoding of Fifty-two Eulophidae Specimens (Hymenoptera) in Northeast China

Tianyu Shao; Weixin Xie; Dan Liu; Xinglong Liu; Sizhu Liu

doi:10.47297/taposatWSP2633-456904.20210205

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The COI DNA Barcoding of Fifty-two Eulophidae Specimens (Hymenoptera) in Northeast China

Volume 2, Issue 5

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The COI DNA Barcoding of Fifty-two Eulophidae Specimens (Hymenoptera) in Northeast China
The COI DNA Barcoding of Fifty-two Eulophidae Specimens (Hymenoptera) in Northeast China
1. Chongqing University of Posts and Telecommunications
2. Institute of Intelligent System and Bioinformatics, College of Intelligent Systems Science and Engineering, Harbin Engineering University
3. Department of Agricultural and Forestry Science and Technology, Chongqing Three Gorges Vocation College
4. Heilongjiang Academy of Agricultural Sciences
Vol. 2, Issue 5, Pages: 35-41(2021)
Published： 2021 ，
DOI：10.47297/taposatWSP2633-456904.20210205
Accepted：

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TIANYU SHAO, WEIXIN XIE, DAN LIU, XINGLONG LIU & SIZHU LIU. (2021). The COI DNA Barcoding of Fifty-two Eulophidae Specimens (Hymenoptera) in Northeast China. Theory and Practice of Science and Technology, 2(5), 35-41..
DOI：

TIANYU SHAO, WEIXIN XIE, DAN LIU, XINGLONG LIU & SIZHU LIU. (2021). The COI DNA Barcoding of Fifty-two Eulophidae Specimens (Hymenoptera) in Northeast China. Theory and Practice of Science and Technology, 2(5), 35-41.. DOI： 10.47297/taposatWSP2633-456904.20210205.

摘要

Abstract

In this study

238 specimens of the family Eulophidae were collected from 6 suction traps in Heilongjiang Province

China. We identified 14 genera and 52 morphospecies before DNA extraction. We amplified and sequenced 60 COI sequences

which were submitted to GenBank

with accession numbers from MG836426 to MG836501 for COI. Intraspecific and interspecific genetic distances were calculated for both COI. The interspecific distances of COI gene was significantly different

with the minimum interspecies genetic distance (6.00%) greater than the maximum intraspecific genetic distance (3.02%)

which indicates a clear barcoding gap between species. sequence similarity and phylogenetic relationship were analyzed by MEGA 7.0 software

indicating that the clustering results of COI gene sequences are basically consistent with morphological classification.

关键词

Keywords

DNA barcodingCOIMolecular identificationEulophidae

references

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