The 28S RNA barcoding of Fifty-two Eulophidae specimens (Hymenoptera) in Northeast China

Tianyu Shao; Weixin Xie; Dan Liu; Xinglong Liu; Sizhu Liu

doi:10.47297/taposatWSP2633-456905.20210206

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The 28S RNA barcoding of Fifty-two Eulophidae specimens (Hymenoptera) in Northeast China

Volume 2, Issue 6

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The 28S RNA barcoding of Fifty-two Eulophidae specimens (Hymenoptera) in Northeast China
The 28S RNA barcoding of Fifty-two Eulophidae specimens (Hymenoptera) in Northeast China
1. Chongqing University of Posts and Telecommunications
2. Institute of Intelligent System and Bioinformatics, College of Intelligent Systems Science and Engineering, Harbin Engineering University
3. Department of Agricultural and Forestry Science and Technology, Chongqing Three Gorges Vocation College
4. Heilongjiang Academy of Agricultural Sciences
Vol. 2, Issue 6, Pages: 28-33(2021)
Published： 2021 ，
DOI：10.47297/taposatWSP2633-456905.20210206
Accepted：

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TIANYU SHAO, WEIXIN XIE, DAN LIU, XINGLONG LIU & SIZHU LIU. (2021). The 28S RNA barcoding of Fifty-two Eulophidae specimens (Hymenoptera) in Northeast China. Theory and Practice of Science and Technology, 2(6), 28-33..
DOI：

TIANYU SHAO, WEIXIN XIE, DAN LIU, XINGLONG LIU & SIZHU LIU. (2021). The 28S RNA barcoding of Fifty-two Eulophidae specimens (Hymenoptera) in Northeast China. Theory and Practice of Science and Technology, 2(6), 28-33.. DOI： 10.47297/taposatWSP2633-456905.20210206.

摘要

Abstract

In this study

238 specimens of the family Eulophidae were collected from 6 suction traps in Heilongjiang Province

China. We identified 14 genera and 52 morpho-species before RNA extraction. We amplified and sequenced 68 28S sequences

which were submitted to GenBank

with accession numbers from MH169010 to MH169101 for 28S. Intraspecific and interspecific genetic distances were calculated for both 28S gene. The 28S is highly variable

the intraspecific genetic distances of many genera exceed the interspecific

the overlap phenomenon is obvious. Futher

sequence similarity and phylogenetic relationship were analyzed by MEGA 7.0 software

indicating that the clustering results of 28S gene sequences are quite different from those of morphological results.

关键词

Keywords

RNA barcoding28SMolecular identificationEulophidae

references

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The COI DNA Barcoding of Fifty-two Eulophidae Specimens (Hymenoptera) in Northeast China

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Weixin Xie Institute of Intelligent System and Bioinformatics, College of Intelligent Systems Science and Engineering, Harbin Engineering University

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