A new antimicrobial synthetic leather material with in situ generation of polyurethane synthetic leather was prepared by combining organic-inorganic nano-hybridization into a wet phase conversion membrane formation method. The antibacterial activity of Staphylococcus aureus

Escherichia coli

Pseudomonas aeruginosa

Micrococcus flavus

the antifungal activity of Aspergillus oryzae and the toxicity of the polyurethane synthetic leather were investigated. The experimental results showed that the antibacterial activity of the polyurethane synthetic leather material increased with increasing polyurethane synthetic leather concentration. Low polyurethane synthetic leather concentrations (≤0.50 wt%) resulted in negligible or limited antimicrobial activity. When the PU synthetic leather concentration was increased to 0.75 and 1.00 wt%

the antibacterial activity of the PU synthetic leather material exceeded 82 and 93%

respectively

and no growth of Aspergillus fumigatus was observed on the surface of the material within 28 days. Cell culture analysis showed that PU synthetic leather had no deleterious effect on the morphology and proliferation rate of normal human dermal fibroblasts

suggesting that it is non-toxic and skin-friendly. Based on these results

polyurethane synthetic leather materials can reduce the risk of microbial contamination while remaining skin friendly to the wearer. More importantly

the organic-inorganic nano-hybrid developed in this study is combined with the formation of wet phase conversion film

which is expected to be applied in industrial scale.