lnfectivity Characteristics of Alfalfa Mosaic Virus lnfectious Clone

Yushu Huang

doi:10.47297/wspciateWSP2633-772X10.20260701

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lnfectivity Characteristics of Alfalfa Mosaic Virus lnfectious Clone

Volume 7, Issue 1

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lnfectivity Characteristics of Alfalfa Mosaic Virus lnfectious Clone
lnfectivity Characteristics of Alfalfa Mosaic Virus lnfectious Clone
Faculty of Science, University of Copenhagen, Copenhagen, 2100, Denmark
Vol. 7, Issue 1, Pages: 50-52(2026)
Online First：12 August 2026，

Published：28 February 2026
DOI：10.47297/wspciateWSP2633-772X10.20260701
Accepted：

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Huang Yushu.lnfectivity Characteristics of Alfalfa Mosaic Virus lnfectious Clone[J].Chinese Industry and Technology of Eco-environment,2026,07(01):50-52.
DOI：

Huang Yushu.lnfectivity Characteristics of Alfalfa Mosaic Virus lnfectious Clone[J].Chinese Industry and Technology of Eco-environment,2026,07(01):50-52. DOI： 10.47297/wspciateWSP2633-772X10.20260701.

摘要

Abstract

Alfalfa mosaic virus (AMV) is a major constraint on alfalfa production in China. Infectious cDNA clones provide a stable system for viral preservation and functional studies. Here

an AMV infectious clone was used to assess pathogenicity and its impact on host growth. Mechanical inoculation showed that Nicotiana benthamiana

but not Medicago sativa

developed typical mosaic symptoms

with a 75% infection rate and a disease index of 33.89 at 10 days post-inoculation. Infected plants exhibited reduced fresh weight and increased dry weight

indicating tissue dehydration

while both total nitrogen and crude protein levels showed no significant change. These results demonstrate that the AMV infectious clone is pathogenic to Nicotiana benthamiana and provides an effective tool for future studies on AMV–host interactions and resistance breeding in alfalfa.

关键词

Keywords

references

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